Reverse Transcription
Reverse Transcription
To the tune of “Teen Idle” by MARINA
[Verse 1]
tRNA will prime
Reverse transcriptase to synthesize
The negative strand of DNA
And degrade the RNA template
Negative DNA complements
The RNA genome 3’ end
Because of this structure
Template exchange can occur
[Chorus]
Only one molecule of DNA
Is produced from two RNA
This process will happen
In the cellular cytoplasm
The LTR will be made
By reverse transcriptase
This genome will then integrate
Into the host DNA
[Verse 2]
RT continues to extend
The negative DNA strand
Polypurine tract on the RNA
Is resistant to RNAse H
The polypurine tract will prime
The positive strand to be synthesized
There are multiple
RTs in a viral particle
[Chorus]
Only one molecule of DNA
Is produced from two RNA
This process will happen
In the cellular cytoplasm
The LTR will be made
By reverse transcriptase
This genome will then integrate
Into the host DNA
DNA
Host DNA
[Bridge]
The polypurine tract and tRNA
Are cleaved by RNAse H
Both strands have primer binding sites
A second template exchange site
Strand displacement synthesis
Displaces one of the strands
A full copy can then be made
Of the positive DNA
[Chorus]
Only one molecule of DNA
Is produced from two RNA
This process will happen
In the cellular cytoplasm
The LTR will be made
By reverse transcriptase
This genome will then integrate
Into the host DNA
Into the host DNA
Into the host DNA
Host DNA
