Reversible Freezing

Reversible Freezing

To the tune of “Hey Jude” by the Beatles

[Verse 1]
Reversible freezing in vertebrates requires strategies
Such as metabolic suppression, cryoprotectants, and INPs
Reversible freezing is more tolerated by amphibians
Because they cope with large change in ion and water concentrations

[Chorus]
Ice nucleating proteins, induce freezing above the animal’s supercooling point
Secreted extracellularly, they will control kinetics and location where ice will form

[Interlude]
Na na na na na na na na

[Verse 2]
Reversible freezing requires cryoprotectant molecules
Increasing unfreezable water to maintain a critical minimum volume

[Chorus]
3 frog species will use glucose, they raise plasma concentration quickly
They also will have glycogen storage within their liver at very high quantities

[Interlude]
Na na na na na na na na

[Verse 3]
Reversible freezing in vertebrates requires strategies
Such as metabolic suppression, cryoprotectants, and INPs

[Outro]
Na na na na na na na na na na na, reversible
Na na na na na na na na na na na, freezing
Na na na na na na na na na na na, reversible
Na na na na na na na na na na na, freezing